Review



prk5 egfp tau plasmid wt 0n4r  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc prk5 egfp tau plasmid wt 0n4r
    Prk5 Egfp Tau Plasmid Wt 0n4r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/10__1016_slash_j__dscb__2025__100236-68-5-14
    Average 93 stars, based on 73 article reviews
    prk5 egfp tau plasmid wt 0n4r - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Tau induces PSD95-nNOS uncoupling and neurovascular dysfunction independent of neurodegeneration
    Article Snippet: Filters were washed 4 times for 2min in 0.5% phosphoric acid, and the remaining radioactivity was quantified in a LS3801 scintillation counter (Beckman Coulter) by the Cherenkov method. .. PSD95 was amplified from C57BL6/J mouse cDNA with primers containing Eco RI (5’- gcacGAATTCatatggactgtctctgtatagtg-3’) and Xho I (5’-gataCTCGAGtcagagtctctctcgggctgg-3’) restriction sites and subsequently cloned into Eco RI/ Xho I-digested pCMV-Myc vector (Clontech). pcDNA3.1-nNOS , pRK5-EGFP-Tau WT and pRK5-EGFP-Tau P301L were kind gifts from Yoichi Osawa (University of Michigan Medical School, Ann Arbor, MI) and Karen Ashe (Addgene plasmid #46904 and #46908; University of Minnesota, Minneapolis, MN), respectively. pcDNA3.1 (Thermo Fisher Scientific) and pEGFP-C3 (Clontech) plasmids were used as empty vector expression controls. ..

    Clone Assay:

    Article Title: Tau induces PSD95-nNOS uncoupling and neurovascular dysfunction independent of neurodegeneration
    Article Snippet: Filters were washed 4 times for 2min in 0.5% phosphoric acid, and the remaining radioactivity was quantified in a LS3801 scintillation counter (Beckman Coulter) by the Cherenkov method. .. PSD95 was amplified from C57BL6/J mouse cDNA with primers containing Eco RI (5’- gcacGAATTCatatggactgtctctgtatagtg-3’) and Xho I (5’-gataCTCGAGtcagagtctctctcgggctgg-3’) restriction sites and subsequently cloned into Eco RI/ Xho I-digested pCMV-Myc vector (Clontech). pcDNA3.1-nNOS , pRK5-EGFP-Tau WT and pRK5-EGFP-Tau P301L were kind gifts from Yoichi Osawa (University of Michigan Medical School, Ann Arbor, MI) and Karen Ashe (Addgene plasmid #46904 and #46908; University of Minnesota, Minneapolis, MN), respectively. pcDNA3.1 (Thermo Fisher Scientific) and pEGFP-C3 (Clontech) plasmids were used as empty vector expression controls. ..

    Plasmid Preparation:

    Article Title: Tau induces PSD95-nNOS uncoupling and neurovascular dysfunction independent of neurodegeneration
    Article Snippet: Filters were washed 4 times for 2min in 0.5% phosphoric acid, and the remaining radioactivity was quantified in a LS3801 scintillation counter (Beckman Coulter) by the Cherenkov method. .. PSD95 was amplified from C57BL6/J mouse cDNA with primers containing Eco RI (5’- gcacGAATTCatatggactgtctctgtatagtg-3’) and Xho I (5’-gataCTCGAGtcagagtctctctcgggctgg-3’) restriction sites and subsequently cloned into Eco RI/ Xho I-digested pCMV-Myc vector (Clontech). pcDNA3.1-nNOS , pRK5-EGFP-Tau WT and pRK5-EGFP-Tau P301L were kind gifts from Yoichi Osawa (University of Michigan Medical School, Ann Arbor, MI) and Karen Ashe (Addgene plasmid #46904 and #46908; University of Minnesota, Minneapolis, MN), respectively. pcDNA3.1 (Thermo Fisher Scientific) and pEGFP-C3 (Clontech) plasmids were used as empty vector expression controls. ..

    Article Title: Tau Misfolding Efficiently Propagates between Individual Intact Hippocampal Neurons
    Article Snippet: .. The following plasmids were used: pRFP-N1, pEGFP-C3 (Clontech); pRK5-EGFP-tau WT and pRK5-EGFP-tau E14 were a gift from Karen Ashe ( Hoover et al., 2010 ; Addgene, plasmids #46904 and #46907); GCaMP6 was a gift from Douglas Kim ( Chen et al., 2013 ; Addgene, plasmid #40753); and R-GECO was a gift from Robert Campbell ( J. Wu et al., 2013 ; Addgene, plasmid #45494). ..

    Article Title: Efficient propagation of misfolded tau between individual neurons occurs in absence of degeneration
    Article Snippet: .. The following plasmids were used: pRFP-N1, pEGFP-C3 (Clontech), pRK5-EGFP-tau WT and pRK5-EGFP-tau E14 were a gift from Karen Ashe (Addgene plasmids #46904 and #46907), GCaMP6 was a gift from Douglas Kim (Addgene plasmid #40753), R-GECO was a gift from Robert Campbell (Addgene plasmid #45494). ..

    Expressing:

    Article Title: Tau induces PSD95-nNOS uncoupling and neurovascular dysfunction independent of neurodegeneration
    Article Snippet: Filters were washed 4 times for 2min in 0.5% phosphoric acid, and the remaining radioactivity was quantified in a LS3801 scintillation counter (Beckman Coulter) by the Cherenkov method. .. PSD95 was amplified from C57BL6/J mouse cDNA with primers containing Eco RI (5’- gcacGAATTCatatggactgtctctgtatagtg-3’) and Xho I (5’-gataCTCGAGtcagagtctctctcgggctgg-3’) restriction sites and subsequently cloned into Eco RI/ Xho I-digested pCMV-Myc vector (Clontech). pcDNA3.1-nNOS , pRK5-EGFP-Tau WT and pRK5-EGFP-Tau P301L were kind gifts from Yoichi Osawa (University of Michigan Medical School, Ann Arbor, MI) and Karen Ashe (Addgene plasmid #46904 and #46908; University of Minnesota, Minneapolis, MN), respectively. pcDNA3.1 (Thermo Fisher Scientific) and pEGFP-C3 (Clontech) plasmids were used as empty vector expression controls. ..



    Similar Products

    prk5 egfp tau plasmid wt 0n4r  (Addgene inc)
    93
    Addgene inc prk5 egfp tau plasmid wt 0n4r
    Prk5 Egfp Tau Plasmid Wt 0n4r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/10__1016_slash_j__dscb__2025__100236-68-5-14
    Average 93 stars, based on 1 article reviews
    prk5 egfp tau plasmid wt 0n4r - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    4r0n tau wt  (Addgene inc)
    93
    Addgene inc 4r0n tau wt
    4r0n Tau Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/pm37730840-89-3-8
    Average 93 stars, based on 1 article reviews
    4r0n tau wt - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    egfp tau wt expressing prk5 vector  (Addgene inc)
    93
    Addgene inc egfp tau wt expressing prk5 vector
    Egfp Tau Wt Expressing Prk5 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/pm34004220-85-2-9
    Average 93 stars, based on 1 article reviews
    egfp tau wt expressing prk5 vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    4 repeat human wt 4r0n tau  (Addgene inc)
    93
    Addgene inc 4 repeat human wt 4r0n tau
    DIPQUO blocks phosphorylation of tau microtubule-associated protein . A , NIH/3T3 fibroblasts were transfected with GFP-tagged human four-repeat WT tau <t>(4R0N)</t> and treated with 500 nM AZD or 10 μM DIPQUO for 24 h and then analyzed by Western blotting for tau expression and activity via phosphorylation of S396. Phospho-S396-tau was quantified as a ratio to total tau in the graph at right. Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 10.12 and p = 0.0119. B , further dose–response Western blotting analysis of tau S396 phosphorylation demonstrated a dose-dependent relationship for DIPQUO, with significant inhibition at the 500 nM dose. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗ p < 0.01 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 7.549 and p = 0.0020. C , phosphorylation of tau S396 and T217 was analyzed after treatment with DIPQUO in the absence or presence of the ERK1/2 inhibitor UO126. Phospho-tau was quantified in the graph as a ratio to total tau, normalized to β-actin, with P-tau-S396 in black and P-tau-T217 in blue . Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Two-way ANOVA yielded F = 1.235 and p = 0.3296. D , depletion of GSK3-β for 24 h with siRNA partially restored S396 phosphorylation of tau after an additional 24 h of 10 μM DIPQUO treatment. The graph at right shows ratio of phospho-S396-tau to total tau, normalized to β-actin protein loading control. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 15.61 and p < 0.0001. Representative images from experiments using at least three biological replicates are shown for all figure panels. AZD, AstraZeneca GSK3-β-specific inhibitor AZD2858; GSK3-β, glycogen synthase kinase 3-beta.
    4 Repeat Human Wt 4r0n Tau, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/pmc08138761-205-4-10
    Average 93 stars, based on 1 article reviews
    4 repeat human wt 4r0n tau - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    126 transfection 127 4r0n egfp tau wt cdna  (Addgene inc)
    93
    Addgene inc 126 transfection 127 4r0n egfp tau wt cdna
    DIPQUO blocks phosphorylation of tau microtubule-associated protein . A , NIH/3T3 fibroblasts were transfected with GFP-tagged human four-repeat WT tau <t>(4R0N)</t> and treated with 500 nM AZD or 10 μM DIPQUO for 24 h and then analyzed by Western blotting for tau expression and activity via phosphorylation of S396. Phospho-S396-tau was quantified as a ratio to total tau in the graph at right. Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 10.12 and p = 0.0119. B , further dose–response Western blotting analysis of tau S396 phosphorylation demonstrated a dose-dependent relationship for DIPQUO, with significant inhibition at the 500 nM dose. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗ p < 0.01 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 7.549 and p = 0.0020. C , phosphorylation of tau S396 and T217 was analyzed after treatment with DIPQUO in the absence or presence of the ERK1/2 inhibitor UO126. Phospho-tau was quantified in the graph as a ratio to total tau, normalized to β-actin, with P-tau-S396 in black and P-tau-T217 in blue . Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Two-way ANOVA yielded F = 1.235 and p = 0.3296. D , depletion of GSK3-β for 24 h with siRNA partially restored S396 phosphorylation of tau after an additional 24 h of 10 μM DIPQUO treatment. The graph at right shows ratio of phospho-S396-tau to total tau, normalized to β-actin protein loading control. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 15.61 and p < 0.0001. Representative images from experiments using at least three biological replicates are shown for all figure panels. AZD, AstraZeneca GSK3-β-specific inhibitor AZD2858; GSK3-β, glycogen synthase kinase 3-beta.
    126 Transfection 127 4r0n Egfp Tau Wt Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/pm31256134-41-0-10
    Average 93 stars, based on 1 article reviews
    126 transfection 127 4r0n egfp tau wt cdna - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    prk5 egfp tau wt  (Addgene inc)
    93
    Addgene inc prk5 egfp tau wt
    DIPQUO blocks phosphorylation of tau microtubule-associated protein . A , NIH/3T3 fibroblasts were transfected with GFP-tagged human four-repeat WT tau <t>(4R0N)</t> and treated with 500 nM AZD or 10 μM DIPQUO for 24 h and then analyzed by Western blotting for tau expression and activity via phosphorylation of S396. Phospho-S396-tau was quantified as a ratio to total tau in the graph at right. Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 10.12 and p = 0.0119. B , further dose–response Western blotting analysis of tau S396 phosphorylation demonstrated a dose-dependent relationship for DIPQUO, with significant inhibition at the 500 nM dose. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗ p < 0.01 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 7.549 and p = 0.0020. C , phosphorylation of tau S396 and T217 was analyzed after treatment with DIPQUO in the absence or presence of the ERK1/2 inhibitor UO126. Phospho-tau was quantified in the graph as a ratio to total tau, normalized to β-actin, with P-tau-S396 in black and P-tau-T217 in blue . Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Two-way ANOVA yielded F = 1.235 and p = 0.3296. D , depletion of GSK3-β for 24 h with siRNA partially restored S396 phosphorylation of tau after an additional 24 h of 10 μM DIPQUO treatment. The graph at right shows ratio of phospho-S396-tau to total tau, normalized to β-actin protein loading control. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 15.61 and p < 0.0001. Representative images from experiments using at least three biological replicates are shown for all figure panels. AZD, AstraZeneca GSK3-β-specific inhibitor AZD2858; GSK3-β, glycogen synthase kinase 3-beta.
    Prk5 Egfp Tau Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+egfp+tau+wt/pRK5-EGFP-Tau+(Plasmid+%2346904)/pmc07896353-286-33-55
    Average 93 stars, based on 1 article reviews
    prk5 egfp tau wt - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    DIPQUO blocks phosphorylation of tau microtubule-associated protein . A , NIH/3T3 fibroblasts were transfected with GFP-tagged human four-repeat WT tau (4R0N) and treated with 500 nM AZD or 10 μM DIPQUO for 24 h and then analyzed by Western blotting for tau expression and activity via phosphorylation of S396. Phospho-S396-tau was quantified as a ratio to total tau in the graph at right. Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 10.12 and p = 0.0119. B , further dose–response Western blotting analysis of tau S396 phosphorylation demonstrated a dose-dependent relationship for DIPQUO, with significant inhibition at the 500 nM dose. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗ p < 0.01 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 7.549 and p = 0.0020. C , phosphorylation of tau S396 and T217 was analyzed after treatment with DIPQUO in the absence or presence of the ERK1/2 inhibitor UO126. Phospho-tau was quantified in the graph as a ratio to total tau, normalized to β-actin, with P-tau-S396 in black and P-tau-T217 in blue . Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Two-way ANOVA yielded F = 1.235 and p = 0.3296. D , depletion of GSK3-β for 24 h with siRNA partially restored S396 phosphorylation of tau after an additional 24 h of 10 μM DIPQUO treatment. The graph at right shows ratio of phospho-S396-tau to total tau, normalized to β-actin protein loading control. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 15.61 and p < 0.0001. Representative images from experiments using at least three biological replicates are shown for all figure panels. AZD, AstraZeneca GSK3-β-specific inhibitor AZD2858; GSK3-β, glycogen synthase kinase 3-beta.

    Journal: The Journal of Biological Chemistry

    Article Title: The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3-beta signaling

    doi: 10.1016/j.jbc.2021.100696

    Figure Lengend Snippet: DIPQUO blocks phosphorylation of tau microtubule-associated protein . A , NIH/3T3 fibroblasts were transfected with GFP-tagged human four-repeat WT tau (4R0N) and treated with 500 nM AZD or 10 μM DIPQUO for 24 h and then analyzed by Western blotting for tau expression and activity via phosphorylation of S396. Phospho-S396-tau was quantified as a ratio to total tau in the graph at right. Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 10.12 and p = 0.0119. B , further dose–response Western blotting analysis of tau S396 phosphorylation demonstrated a dose-dependent relationship for DIPQUO, with significant inhibition at the 500 nM dose. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗ p < 0.01 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 7.549 and p = 0.0020. C , phosphorylation of tau S396 and T217 was analyzed after treatment with DIPQUO in the absence or presence of the ERK1/2 inhibitor UO126. Phospho-tau was quantified in the graph as a ratio to total tau, normalized to β-actin, with P-tau-S396 in black and P-tau-T217 in blue . Values are reported as the means ± SD; ∗ p < 0.05 in unpaired two-tailed Student's t test. Two-way ANOVA yielded F = 1.235 and p = 0.3296. D , depletion of GSK3-β for 24 h with siRNA partially restored S396 phosphorylation of tau after an additional 24 h of 10 μM DIPQUO treatment. The graph at right shows ratio of phospho-S396-tau to total tau, normalized to β-actin protein loading control. Values are reported as the means ± SD; ∗ p < 0.05 and ∗∗∗∗ p < 0.0001 in unpaired two-tailed Student's t test. Ordinary one-way ANOVA yielded F = 15.61 and p < 0.0001. Representative images from experiments using at least three biological replicates are shown for all figure panels. AZD, AstraZeneca GSK3-β-specific inhibitor AZD2858; GSK3-β, glycogen synthase kinase 3-beta.

    Article Snippet: An expression construct containing 4-repeat human WT 4R0N tau (pRK5-EGFP-tau, Addgene 46904) was transfected into 70% confluent NIH/3T3 cells using Lipofectamine LTX Plus reagent (Invitrogen 15338-100), and DIPQUO or inhibitor treatments were performed simultaneously.

    Techniques: Phospho-proteomics, Transfection, Western Blot, Expressing, Activity Assay, Two Tailed Test, Inhibition, Control